primer extension

The mRNA transcriptional start site was determined by primer extension , which located at nt - 50 in the high conserved 12-mer nucleotide domain , similar as AcNPV .

采用引物延伸法，对ApNPV核多角体蛋白mRNA转录起始点进行了测定，确定其位于该基因调控序列12个核苷酸高保守区的nt&50位点与AcNPV相似。

To detect p53 SNPs , the method of an arrayed primer extension based on oligonucleotide microarray was developed . METHODS : Twelve extension primers were designed in exon 3 of p53 gene .

本文应用寡核苷酸阵列引物延伸法对人类肿瘤相关性最高的基因&p53基因进行SNPs的平行检测。

Analysis of several genes using primer extension preamplification based on individual cell

单细胞引物延伸预扩增分析多个基因

Electrochemical Detection for SNP Genotyping Based on Primer Extension Reaction

基于引物延伸反应进行SNP基因分型的电化学方法

Single Base Discrimination Using Terminal Labeled Primer Extension

末端标记引物延伸反应对核酸单碱基辨认的实验研究

Detection of fetal DNA using primer extension preamplification followed by nested PCR

应用引物延伸预扩增反应技术检测孕妇血浆中的胎儿DNA

Random primer extension radiolabeled DNA probe and its application

随机引物法标记放射同位素DNA探针及其应用

Application of Oligonucleotide Microarray Primer Extension to Detection of p53 Single Nucleotide Polymorphisms

寡核苷酸阵列引物延伸技术在p53基因突变检测中的应用

Detection of multiple loci in single cell by primer extension preamplification and nest PCR

单细胞扩增前引物延伸多基因位点检测研究

The primer extension experiment showed the aggregation of DNA template by photooxydation .

引物延伸反应表明DNA分子发生聚合。

Different with genechip based on hybridization detection , the chip we developed is based on arrayed primer extension .

和以往基于杂交检测原理的基因芯片不同，我们探讨了基于引物延伸原理的芯片检测方法。

Establishment and Application of Research Model for Screening the K-ras Alleles by MALDI-TOF-MS Coupled with Primer Extension Reaction Method

MALDI-TOF-MS结合引物延伸反应检测K-ras基因型方法的建立

Identification of fetal nucleated erythrocytes in maternal blood using short tandem repeat typing after primer extension preamplification

引物延伸预扩增后STR分型在鉴定母血中胎儿有核红细胞的应用

Accurate and rapid prenatal diagnosis of β - thalassemia by a multiplex primer extension and denaturing high performance liquid chromatography technique

引物延伸变性高效液相色谱产前诊断β-地中海贫血

Objective To study the effect of modified improved primer extension preamplification ( IPEP ) on STR analysis of trace DNA .

目的探讨改良扩增前引物延伸（IPEP）法对痕量DNA样本STR检测分型的效果。

Genotyping analysis of multiple loci in single cell by primer extension preamplification and degenerate oligonucleotide primed - PCR

PEP、DOP-PCR单细胞多基因位点检测研究

The signal peptide of IgG κ light chain was fused to N-terminal of the A β 15 gene by primer extension .

在N端引物延伸加上IgGκ轻链信号肽序列；

The PRINS reaction is based on the use of a DNA polymerase and labeled nucleotide in an in situ primer extension reaction .

PRINS反应的基本物是DNA聚合酶和引物原位扩展反应中的标引核苷。缩氨酸核酸探针是合成的不带电聚酰胺主链的DNA类似物。

One distinct band was detected in each primer extension reaction with total RNA extracted from wheat leaves and the primers specific for the wheat genes .

利用两种基因的特定引物及总RNA进行的引物延伸反应产物在电泳的凝胶上均呈现一条十分清楚的带，表明这两种多拷贝小麦基因的转录分别起始于一个核苷酸。

To quantify the products of single nucleotide primer extension ( SNuPE ), the single strain oligonucleotides were separated by High Performance Liquid Chromatography ( HPLC ) .

建立用普通高效液相色谱仪检测单链寡聚核苷酸的方法，为检测单碱基引物延伸反应的产物提供定量分析基础。

With polymerase and the dNTPs , the replication of the single-stranded domain of hairpin-probe triggers the process of primer extension .

在聚合酶与dNTPs的作用下，复制发卡探针的单链部分引发了引物延长进程。

The switching characteristics of the primer extension reaction was increased by introducing an artificially mismatched base into the third position from the 3 ′ - terminus of the primers .

修饰引物的3′端第三个碱基处人为导入一个错配碱基来改善引物延伸反应的开关特性。

Primer extension preamplification ( PEP ) and ploymerase chain reaction ( PCR ) based on single cell were adopted to detect SRY gene . Fluorescence in situ hybridization was also applied .

采用单细胞PEP-PCR技术检测SRY基因，并进行荧光原位杂交分析。

Methods Using primer extension preamplification ( PEP ) and nested polymerase chain reaction ( PCR ), a normal male SRY gene in maternal plasma DNA was detected in 65 gravidas .

方法采用引物延伸预扩增（PEP）法及巢式PCR技术对65例孕妇外周血血浆DNA进行正常男性SRY基因检测。

Non-specific primer extension was decreased remarkably after introducing a mismatch at the third or fourth 3 ′ - terminal base of allele specific primers , and the stringency of PCR reaction was cut down .

两条特异性上游引物3'端第3或第4位碱基引入错配后非特异性延伸显著减少，且对PCR反应条件的严格性要求明显降低。

The purified RNA is ready for use in standard and downstream applications such as RT PCR , polyA + RNA selection , differential display , Northern dot and slot blotting , primer extension , cDNA synthesis , expression array and expression chip analysis .

通过此方法纯化的RNA可用于各种标准的下游技术，如RT-PCR、polyA+RNA选择、差异显示技术、Northern点杂交和狭线杂交、引物延伸、cDNA合成、陈列表达分析和表达芯片分析等。

We determined the transcription start site ( TSS ) of hb1f ( nr5a2 ) gene via RT-PCR and primer extension and then identified the promoter of hb1f ( nr5a2 ) gene , which exhibited high hepatocyte specific activity .

首先利用RT-PCR和引物延伸方法确定了hb1f（nr5a2）基因的转录起始位点，并通过序列缺失方法鉴定出hb1f（nr5a2）基因的核心启动子。

An improved procedure for primer extension analysis was used to identify the transcription start site of a phenylalanine ammonia-lyase ( PAL ) gene and a chitinase gene from wheat . This procedure requires less amount of isotope , a Sephadex G-25 column and highly stringent hybridization conditions .

本文应用改良的引物延伸法，鉴定了一个小麦的苯丙氨酸解氨酶和几丁质酶基因的转录起始点。这种方法只需少量同位素，SephadexG－25柱和严格的杂交条件。

Core primer combination was an extension of primer combination method , data from different laboratories could be compared and integrated by using a set of fixed core primer combination and it would lead to standardization of maize DNA fingerprint analysis .

核心引物组合法是引物组合法的扩展，不同实验室通过使用固定的一套核心引物组合，其指纹图谱可以相互比较和整合，为玉米DNA指纹图谱分析的标准化奠定基础。